Silicon influences the localization and expression of Phytophthora sojae effectors in interaction with soybean.
Identifieur interne : 000058 ( Main/Exploration ); précédent : 000057; suivant : 000059Silicon influences the localization and expression of Phytophthora sojae effectors in interaction with soybean.
Auteurs : Aliyeh Rasoolizadeh [Canada] ; Parthasarathy Santhanam [Canada] ; Caroline Labbé [Canada] ; Sheelavanta Matha Shivaraj [Canada] ; Hugo Germain [Canada] ; Richard R. Bélanger [Canada]Source :
- Journal of experimental botany [ 1460-2431 ] ; 2020.
Abstract
In plant-pathogen interactions, expression and localization of effectors in the aqueous apoplastic region play a crucial role in the establishment or suppression of pathogen development. Silicon (Si) has been shown to protect plants in several host-pathogen interactions but its mode of action remains a source of debate. Its deposition in the apoplastic area of plant cells suggests it might interfere with receptor/effector recognition. In this study, soybean plants treated or not with Si were inoculated with Phytophthora sojae and differences in the ensuing infection process assessed through different microscopy techniques, transcript analysis of effector and defense genes, and effector (Avr6) localization through immunolocalization and fluorescence labeling. In plants grown without Si, results showed the rapid (4 dpi) host recognition by P. sojae through the development of haustorium-like bodies, followed by expression and release of effectors into the apoplastic region. By contrast, Si treatment resulted in limited pathogen development, and significantly lower expression and presence of Avr6 in the apoplastic region. Based on immunolocalization and quantification of Avr6 through fluorescence labeling, our results suggest that the presence of Si in the apoplast interferes with host recognition and/or limits receptor-effector interactions, which leads to an incompatible interaction.
DOI: 10.1093/jxb/eraa101
PubMed: 32090252
Affiliations:
- Canada
- Québec
- Québec (ville), Trois-Rivières
- Université Laval, Université du Québec à Trois-Rivières
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Silicon influences the localization and expression of Phytophthora sojae effectors in interaction with soybean.</title><author><name sortKey="Rasoolizadeh, Aliyeh" sort="Rasoolizadeh, Aliyeh" uniqKey="Rasoolizadeh A" first="Aliyeh" last="Rasoolizadeh">Aliyeh Rasoolizadeh</name><affiliation wicri:level="4"><nlm:affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6</wicri:regionArea><orgName type="university">Université Laval</orgName><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Santhanam, Parthasarathy" sort="Santhanam, Parthasarathy" uniqKey="Santhanam P" first="Parthasarathy" last="Santhanam">Parthasarathy Santhanam</name><affiliation wicri:level="4"><nlm:affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6</wicri:regionArea><orgName type="university">Université Laval</orgName><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Labbe, Caroline" sort="Labbe, Caroline" uniqKey="Labbe C" first="Caroline" last="Labbé">Caroline Labbé</name><affiliation wicri:level="4"><nlm:affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6</wicri:regionArea><orgName type="university">Université Laval</orgName><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Shivaraj, Sheelavanta Matha" sort="Shivaraj, Sheelavanta Matha" uniqKey="Shivaraj S" first="Sheelavanta Matha" last="Shivaraj">Sheelavanta Matha Shivaraj</name><affiliation wicri:level="4"><nlm:affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6</wicri:regionArea><orgName type="university">Université Laval</orgName><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Germain, Hugo" sort="Germain, Hugo" uniqKey="Germain H" first="Hugo" last="Germain">Hugo Germain</name><affiliation wicri:level="4"><nlm:affiliation>Département de chimie, biochimie et physique, Université du Québec à Trois-Rivières, Trois-Rivières, Québec, Québec G9A 5H7 Canada.</nlm:affiliation><orgName type="university">Université du Québec à Trois-Rivières</orgName><country>Canada</country><placeName><settlement type="city">Trois-Rivières</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Belanger, Richard R" sort="Belanger, Richard R" uniqKey="Belanger R" first="Richard R" last="Bélanger">Richard R. Bélanger</name><affiliation wicri:level="4"><nlm:affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6</wicri:regionArea><orgName type="university">Université Laval</orgName><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32090252</idno><idno type="pmid">32090252</idno><idno type="doi">10.1093/jxb/eraa101</idno><idno type="wicri:Area/Main/Corpus">000264</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000264</idno><idno type="wicri:Area/Main/Curation">000264</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000264</idno><idno type="wicri:Area/Main/Exploration">000264</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Silicon influences the localization and expression of Phytophthora sojae effectors in interaction with soybean.</title><author><name sortKey="Rasoolizadeh, Aliyeh" sort="Rasoolizadeh, Aliyeh" uniqKey="Rasoolizadeh A" first="Aliyeh" last="Rasoolizadeh">Aliyeh Rasoolizadeh</name><affiliation wicri:level="4"><nlm:affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6</wicri:regionArea><orgName type="university">Université Laval</orgName><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Santhanam, Parthasarathy" sort="Santhanam, Parthasarathy" uniqKey="Santhanam P" first="Parthasarathy" last="Santhanam">Parthasarathy Santhanam</name><affiliation wicri:level="4"><nlm:affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6</wicri:regionArea><orgName type="university">Université Laval</orgName><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Labbe, Caroline" sort="Labbe, Caroline" uniqKey="Labbe C" first="Caroline" last="Labbé">Caroline Labbé</name><affiliation wicri:level="4"><nlm:affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6</wicri:regionArea><orgName type="university">Université Laval</orgName><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Shivaraj, Sheelavanta Matha" sort="Shivaraj, Sheelavanta Matha" uniqKey="Shivaraj S" first="Sheelavanta Matha" last="Shivaraj">Sheelavanta Matha Shivaraj</name><affiliation wicri:level="4"><nlm:affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6</wicri:regionArea><orgName type="university">Université Laval</orgName><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Germain, Hugo" sort="Germain, Hugo" uniqKey="Germain H" first="Hugo" last="Germain">Hugo Germain</name><affiliation wicri:level="4"><nlm:affiliation>Département de chimie, biochimie et physique, Université du Québec à Trois-Rivières, Trois-Rivières, Québec, Québec G9A 5H7 Canada.</nlm:affiliation><orgName type="university">Université du Québec à Trois-Rivières</orgName><country>Canada</country><placeName><settlement type="city">Trois-Rivières</settlement><region type="state">Québec</region></placeName></affiliation></author><author><name sortKey="Belanger, Richard R" sort="Belanger, Richard R" uniqKey="Belanger R" first="Richard R" last="Bélanger">Richard R. Bélanger</name><affiliation wicri:level="4"><nlm:affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</nlm:affiliation><country xml:lang="fr">Canada</country><wicri:regionArea>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6</wicri:regionArea><orgName type="university">Université Laval</orgName><placeName><settlement type="city">Québec (ville)</settlement><region type="state">Québec</region></placeName></affiliation></author></analytic><series><title level="j">Journal of experimental botany</title><idno type="eISSN">1460-2431</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In plant-pathogen interactions, expression and localization of effectors in the aqueous apoplastic region play a crucial role in the establishment or suppression of pathogen development. Silicon (Si) has been shown to protect plants in several host-pathogen interactions but its mode of action remains a source of debate. Its deposition in the apoplastic area of plant cells suggests it might interfere with receptor/effector recognition. In this study, soybean plants treated or not with Si were inoculated with Phytophthora sojae and differences in the ensuing infection process assessed through different microscopy techniques, transcript analysis of effector and defense genes, and effector (Avr6) localization through immunolocalization and fluorescence labeling. In plants grown without Si, results showed the rapid (4 dpi) host recognition by P. sojae through the development of haustorium-like bodies, followed by expression and release of effectors into the apoplastic region. By contrast, Si treatment resulted in limited pathogen development, and significantly lower expression and presence of Avr6 in the apoplastic region. Based on immunolocalization and quantification of Avr6 through fluorescence labeling, our results suggest that the presence of Si in the apoplast interferes with host recognition and/or limits receptor-effector interactions, which leads to an incompatible interaction.</div></front></TEI><pubmed><MedlineCitation Status="Publisher" Owner="NLM"><PMID Version="1">32090252</PMID><DateRevised><Year>2020</Year><Month>02</Month><Day>24</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1460-2431</ISSN><JournalIssue CitedMedium="Internet"><PubDate><Year>2020</Year><Month>Feb</Month><Day>24</Day></PubDate></JournalIssue><Title>Journal of experimental botany</Title><ISOAbbreviation>J Exp Bot</ISOAbbreviation></Journal><ArticleTitle>Silicon influences the localization and expression of Phytophthora sojae effectors in interaction with soybean.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">eraa101</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1093/jxb/eraa101</ELocationID><Abstract><AbstractText>In plant-pathogen interactions, expression and localization of effectors in the aqueous apoplastic region play a crucial role in the establishment or suppression of pathogen development. Silicon (Si) has been shown to protect plants in several host-pathogen interactions but its mode of action remains a source of debate. Its deposition in the apoplastic area of plant cells suggests it might interfere with receptor/effector recognition. In this study, soybean plants treated or not with Si were inoculated with Phytophthora sojae and differences in the ensuing infection process assessed through different microscopy techniques, transcript analysis of effector and defense genes, and effector (Avr6) localization through immunolocalization and fluorescence labeling. In plants grown without Si, results showed the rapid (4 dpi) host recognition by P. sojae through the development of haustorium-like bodies, followed by expression and release of effectors into the apoplastic region. By contrast, Si treatment resulted in limited pathogen development, and significantly lower expression and presence of Avr6 in the apoplastic region. Based on immunolocalization and quantification of Avr6 through fluorescence labeling, our results suggest that the presence of Si in the apoplast interferes with host recognition and/or limits receptor-effector interactions, which leads to an incompatible interaction.</AbstractText><CopyrightInformation>© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rasoolizadeh</LastName><ForeName>Aliyeh</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Santhanam</LastName><ForeName>Parthasarathy</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Labbé</LastName><ForeName>Caroline</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Shivaraj</LastName><ForeName>Sheelavanta Matha</ForeName><Initials>SM</Initials><AffiliationInfo><Affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Germain</LastName><ForeName>Hugo</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Département de chimie, biochimie et physique, Université du Québec à Trois-Rivières, Trois-Rivières, Québec, Québec G9A 5H7 Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bélanger</LastName><ForeName>Richard R</ForeName><Initials>RR</Initials><AffiliationInfo><Affiliation>Département de Phytologie, Université Laval, Québec, Québec, Canada G1V 0A6, Canada.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>02</Month><Day>24</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Exp Bot</MedlineTA><NlmUniqueID>9882906</NlmUniqueID><ISSNLinking>0022-0957</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Effectors (Avr6)</Keyword><Keyword MajorTopicYN="N">Fluorescence labeling</Keyword><Keyword MajorTopicYN="N">Glycine max</Keyword><Keyword MajorTopicYN="N">ImmunolocalizationPhytophthora sojae</Keyword><Keyword MajorTopicYN="N">Plant receptors (Rps6)</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2020</Year><Month>01</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>2</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2020</Year><Month>2</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2020</Year><Month>2</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>aheadofprint</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32090252</ArticleId><ArticleId IdType="pii">5753418</ArticleId><ArticleId IdType="doi">10.1093/jxb/eraa101</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Québec</li></region><settlement><li>Québec (ville)</li><li>Trois-Rivières</li></settlement><orgName><li>Université Laval</li><li>Université du Québec à Trois-Rivières</li></orgName></list><tree><country name="Canada"><region name="Québec"><name sortKey="Rasoolizadeh, Aliyeh" sort="Rasoolizadeh, Aliyeh" uniqKey="Rasoolizadeh A" first="Aliyeh" last="Rasoolizadeh">Aliyeh Rasoolizadeh</name></region><name sortKey="Belanger, Richard R" sort="Belanger, Richard R" uniqKey="Belanger R" first="Richard R" last="Bélanger">Richard R. Bélanger</name><name sortKey="Germain, Hugo" sort="Germain, Hugo" uniqKey="Germain H" first="Hugo" last="Germain">Hugo Germain</name><name sortKey="Labbe, Caroline" sort="Labbe, Caroline" uniqKey="Labbe C" first="Caroline" last="Labbé">Caroline Labbé</name><name sortKey="Santhanam, Parthasarathy" sort="Santhanam, Parthasarathy" uniqKey="Santhanam P" first="Parthasarathy" last="Santhanam">Parthasarathy Santhanam</name><name sortKey="Shivaraj, Sheelavanta Matha" sort="Shivaraj, Sheelavanta Matha" uniqKey="Shivaraj S" first="Sheelavanta Matha" last="Shivaraj">Sheelavanta Matha Shivaraj</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PhytophthoraV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000058 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000058 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PhytophthoraV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:32090252
|texte= Silicon influences the localization and expression of Phytophthora sojae effectors in interaction with soybean.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:32090252" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PhytophthoraV1
| This area was generated with Dilib version V0.6.38. |  |